Assignment Detail:- Practical - Bacteriology
Gram StainRefer to Practical 2, Part 5 for method-
Catalase Production1- Place a glass slide, your cultured plate and your tootpick container within the zone of sterility-2- Select a colony from a previously cultured plate using a toothpick- Take care to avoid agar- Smear this onto the surface of a glass slide3- Place a drop of catalase reagent -hydrogen peroxide- onto the deposited bacteria on the glass slide-4- Observe the bacteria for the production of bubbles
Note: If the organism is catalase positive, bubbles will develop around the inoculum almost immediately-
Oxidase Production1- Place a filter paper onto the surface of a glass slide-2- Add 2 to 3 drops of the oxidase reagent to the filter paper3- Place the previously cultured agar plate upside down on the bench so that the lid is on the bench-4- Hold the plate within the zone of sterility leaving the lid on the bench-5- Using a sterile toothpick pick a single colony of bacteria from a previously cultured plate- Do not use a metal loop-6- Replace the agar plate lid7- Smear the bacterial colony onto the filter paper on the surface of a glass slide
Motility TestRefer to Practical 2, Part 4 for methods- Use the broth cultures provided to complete this test- Bacteria grown on solid media often demonstrate reduced motility-
Acid-Fast Stain -Ziehl-Neelsen-Observe the slide or laminate of Mycobacterium provided as a demonstration and and illustrate, label and describe your observations-
Spore StainEndospores may be observed during the interpretation of a Gram stain, however a special spore stain will demonstrate bacterial spores much more clearly- Cells will stain red while the spores will stain green-
Observe the spore stain preparation or laminate provided as a demonstration and illustrate, label and describe your observations-
Attachment:- Microbial Diversity-rar
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